Apprentice in Biochemistry/Proteomic - Apprenti(e) en Biochimie/proteomique (H/F)

Apprentice in Biochemistry/Proteomic - Apprenti(e) en Biochimie/proteomique (H/F)

General Summary :

The candidate will develop a PALMS method aimed at identifying the binding site of a compound to its target protein in a cellular context. Now, we can apply the PALMS workflow on pure recombinant proteins. But for transmembrane proteins like GPCRs or ion channels, this method is more challenging. Being able to apply PALMS in a native context would be a great advantage

In collaboration with MS team and biochemistry team you will participate to the development of new probes allowing the binding site identification of a target of interest. Different approaches of biochemistry will be first applied, after different steps of validation, the assay will be done with a MS read-out to determine the peptide sequence and the amino acid where the binding occurs

Missions and responsibilities:

• Collect, understand and summarize a publication

• Contribute to proteomic studies to identify the binding site of a known target from a recombinant model and in a cell model.

• Identify and implement novel approaches in biochemistry compatible with proteomic analysis based on data from the literature, in collaboration with the tutor.

• Interpret and exploit experimental results in the biological context in coordination with proteomics, bioinformatics teams.

• Participate in the development and implementation of new methodologies and strategies applied to sample preparation for proteomics.

• Communicate effectively internally through written and oral communication and presentations.

• Demonstrate excellent organization/multitasking, communication and analytical skills with ability to effectively present scientific findings to project team

• Demonstrate strong problem-solving capabilities.

• Closely collaborate with other proteomic team

Knowledge, skills and abilities :

•  Excellent communication and interpersonal skills

• Knowledge in cell culture using cell lines, primary cells, bacteria, or yeast.

• Knowledge in proteomics sample preparation for mass spectrometry analysis.

• Knowledge with SDS-PAGE and Western blotting.

• Scientific rigor and excellent analytical capabilities.

• Capability to work with interdisciplinary teams.

Experience and education :

• Master/ingenior student with a background in chemistry and proteomics

Duration: ~1 year

FR : Dans le cadre de sa politique Diversité, Evotec étudie, à compétences égales, toutes les candidatures dont celles des personnes en situation de handicap.

ENG : In the frame of our Diversity policy, Evotec considers, with equal competences, all applications including people with disabilities.